Hemin reconstitutes proton extrusion in an H(+)-ATPase-negative mutant of Lactococcus lactis.
نویسندگان
چکیده
H(+)-ATPase is considered essential for growth of Lactococcus lactis. However, media containing hemin restored the aerobic growth of an H(+)-ATPase-negative mutant, suggesting that hemin complements proton extrusion. We show that inverted membrane vesicles prepared from hemin-grown L. lactis cells are capable of coupling NADH oxidation to proton translocation.
منابع مشابه
The efflux of a fluorescent probe is catalyzed by an ATP-driven extrusion system in Lactococcus lactis.
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ورودعنوان ژورنال:
- Journal of bacteriology
دوره 183 22 شماره
صفحات -
تاریخ انتشار 2001